1VJE
Crystal structure of a autoinducer-2 synthesis protein with bound selenomethionine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.548, 81.917, 49.327 |
| Unit cell angles | 90.00, 102.81, 90.00 |
Refinement procedure
| Resolution | 36.040 - 1.640 |
| Rwork | 0.188 |
| R-free | 0.21500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.379 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (4.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.040 | 1.730 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.102 | 0.191 |
| Number of reflections | 40542 | |
| <I/σ(I)> | 13.6 | 7.8 |
| Completeness [%] | 98.9 | 98.9 |
| Redundancy | 7.4 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
