1VJD
Structure of pig muscle PGK complexed with ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-12-10 |
| Detector | RIGAKU RAXIS II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.200, 106.400, 51.100 |
| Unit cell angles | 90.00, 97.80, 90.00 |
Refinement procedure
| Resolution | 34.000 - 1.900 |
| R-factor | 0.1917 |
| Rwork | 0.187 |
| R-free | 0.23510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kf0 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.150 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.000 | 2.010 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.087 | |
| Number of reflections | 26788 | |
| Completeness [%] | 92.8 | 0.583 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 287 | NaK phosphate, DTT, Na-azide, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 287K |
