1VID
CATECHOL O-METHYLTRANSFERASE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.5 |
| Synchrotron site | SRS |
| Beamline | PX9.5 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-08 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 51.300, 51.300, 168.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.194 |
| Rwork | 0.194 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 23.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 15.000 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.083 |
| Total number of observations | 158331 * |
| Number of reflections | 16005 |
| Completeness [%] | 85.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 * | 22 * | Vidgren, J., (1991) Proteins: Struct.,Funct., Genet., 11, 233. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | ||
| 2 | 1 | drop | PEG6000 | 8 (%(w/v)) | |
| 3 | 1 | drop | MES | 50 (mM) | |
| 4 | 1 | reservoir | sodium chloride | 0.5 (M) |
