1VI7
Crystal structure of an hypothetical protein
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9795, 0.9795 |
| Spacegroup name | H 3 |
| Unit cell lengths | 148.749, 148.749, 35.602 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.190 - 2.800 |
| R-factor | 0.2591 * |
| Rwork | 0.261 |
| R-free | 0.31600 * |
| Structure solution method | Se-Met MAD phasing |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.400 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (4.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.190 | 2.700 |
| High resolution limit [Å] | 2.800 * | 2.560 |
| Rmerge | 0.104 * | 0.485 * |
| Total number of observations | 81478 * | |
| Number of reflections | 7231 * | |
| <I/σ(I)> | 14.8 | 4.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.3 | 11.3 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 9 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 10 | 1 | reservoir | beta-mercaptoethanol | 28.4 (mM) | |
| 2 | 1 | drop | beta-mercaptoethanol | 1.0 (mM) | |
| 3 | 1 | drop | 150 (mM) | ||
| 4 | 1 | drop | HEPES | 10 (mM) | pH7.5 |
| 5 | 1 | drop | methionine | 10 (mM) | |
| 6 | 1 | drop | glycerol | 10 (%) | |
| 7 | 1 | reservoir | MES | 150 (mM) | pH6.5 |
| 8 | 1 | reservoir | ammonium sulfate | 150 (mM) | |
| 9 | 1 | reservoir | PEG5000 MME | 30 (%) |
