1VI4
Crystal structure of Regulator of ribonuclease activity A protein 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9795 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 115.330, 115.330, 94.585 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.060 - 1.870 |
| Rwork | 0.229 |
| R-free | 0.28200 |
| Structure solution method | Se-Met SAD phasing |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.500 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (4.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.060 | 1.970 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.112 | 0.839 |
| Number of reflections | 20168 | |
| <I/σ(I)> | 19 | 4 |
| Completeness [%] | 99.6 | 97.6 |
| Redundancy | 20.8 | 13.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 10 (mM) | pH7.5 |
| 2 | 1 | drop | 150 (mM) | ||
| 3 | 1 | drop | methionine | 10 (mM) | |
| 4 | 1 | drop | glycerol | 10 (%) | |
| 5 | 1 | drop | dithiothreitol | 5 (mM) | |
| 6 | 1 | drop | protein | 10 (mg/ml) |
