1VHI
EPSTEIN BARR VIRUS NUCLEAR ANTIGEN-1 DNA-BINDING DOMAIN, RESIDUES 470-607
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 297 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-11-07 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.990, 69.080, 70.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.500 |
| R-factor | 0.179 |
| Rwork | 0.179 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.017 |
| RMSD bond angle | 3.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | X-PLOR (3.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.080 | |
| Total number of observations | 83324 * | |
| Number of reflections | 10110 | |
| <I/σ(I)> | 19.7 | 5.5 |
| Completeness [%] | 95.9 | 91.3 |
| Redundancy | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 * | HANGING DROP VAPOR DIFFUSION, 50 MM MES PH 6.0 AND 100 MM NACL. SEE REFERENCE 1 FOR MORE DETAILS., vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7-8 (mg/ml) | |
| 2 | 1 | drop | HEPES | 1 (mM) | |
| 3 | 1 | drop | 500 (mM) | ||
| 4 | 1 | drop | DTT | 10 (mM) | |
| 5 | 1 | reservoir | MES | 50 (mM) | |
| 6 | 1 | reservoir | 0-150 (mM) | ||
| 7 | 1 | reservoir | DTT | 10 (mM) |
