1VGR
Formyl-CoA transferase mutant Asp169 to Glu
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2003-09-02 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.812 |
| Spacegroup name | I 4 |
| Unit cell lengths | 150.042, 150.042, 99.645 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.800 - 2.100 |
| R-factor | 0.18409 |
| Rwork | 0.182 |
| R-free | 0.22006 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1p5r |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.376 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 61094 | |
| <I/σ(I)> | 12.7 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.2 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG 4000, Hepes, Mg chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
