1VGQ
Formyl-CoA transferase mutant Asp169 to Ala
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2003-09-02 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.812 |
| Spacegroup name | I 4 |
| Unit cell lengths | 151.116, 151.116, 99.593 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.170 - 2.130 |
| R-factor | 0.172 |
| Rwork | 0.171 |
| R-free | 0.20190 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1p5r |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.404 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.170 | 2.240 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Number of reflections | 61071 | |
| <I/σ(I)> | 13.7 | 2.2 |
| Completeness [%] | 98.2 | 81.9 |
| Redundancy | 4.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG 4000, Mg chloride, Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
