1VGM
Crystal Structure of an Isozyme of Citrate Synthase from Sulfolbus tokodaii strain7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2003-12-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.870, 94.170, 77.620 |
| Unit cell angles | 90.00, 97.22, 90.00 |
Refinement procedure
| Resolution | 30.300 - 2.000 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o7x |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.900 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.300 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 | 0.198 |
| Number of reflections | 57694 | |
| <I/σ(I)> | 3.8 | |
| Completeness [%] | 95.4 | 97.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Ammonium Sulfate, HEPES, PEG400, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
