1VG8
GPPNHP-Bound Rab7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-06-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 1 |
| Unit cell lengths | 57.058, 57.071, 74.310 |
| Unit cell angles | 71.73, 71.69, 77.65 |
Refinement procedure
| Resolution | 19.560 - 1.700 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Rab7 from REP1:Rab7 complex (PDB code 1VG0) |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.800 |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.600 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 88795 | |
| <I/σ(I)> | 9.95 | 3.04 |
| Completeness [%] | 96.1 | 95 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 298 | 24% PEG 3350, 0.25M Ammonium Phosphate, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
