1VFZ

Crystal Structure of the Kif1A Motor Domain Complexed With ADP-Mg-VO4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	93
Detector technology	CCD
Collection date	2003-06-27
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	41.471, 51.805, 156.992
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.360 - 2.240
R-factor	0.215
Rwork	0.215
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1i5s
RMSD bond length	0.009
RMSD bond angle	1.800
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.320
High resolution limit [Å]	2.240	2.240
Number of reflections	17147
Completeness [%]	99.5	94.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	297	PEG4000, Sodium acetate, Tris-HCl, Xylitol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 297.0K