1VFL
Adenosine deaminase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL24XU |
| Synchrotron site | SPring-8 |
| Beamline | BL24XU |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-03-25 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 0.8266 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 73.726, 73.726, 134.013 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.430 - 1.800 |
| R-factor | 0.24 |
| Rwork | 0.230 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNX (2002) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 28.430 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 34175 |
| Completeness [%] | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | PEG4000, 2-propanol, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
