1VFH
Crystal structure of alanine racemase from D-cycloserine producing Streptomyces lavendulae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 2003-05-22 |
Detector | RIGAKU RAXIS |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 83.980, 63.380, 85.950 |
Unit cell angles | 90.00, 120.14, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.000 |
R-factor | 0.197 |
Rwork | 0.197 |
R-free | 0.25200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1sft |
RMSD bond length | 0.005 |
RMSD bond angle | 1.100 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.111 | 0.609 |
Number of reflections | 24889 | |
<I/σ(I)> | 6.7 | 1.5 |
Completeness [%] | 94.0 | 86.9 |
Redundancy | 2.9 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | Ammonium sulfate, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |