1VFD

HUMAN LACTOFERRIN, N-TERMINAL LOBE MUTANT WITH ARG 121 REPLACED BY GLU (R121E)

Experimental procedure

Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Detector technology	IMAGE PLATE
Spacegroup name	C 2 2 21
Unit cell lengths	98.100, 78.900, 113.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.204 *
Rwork	0.204
R-free	0.31700 *
RMSD bond length	0.016 *
RMSD bond angle	2.000 *
Data reduction software	DENZO
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.500 *	2.500 *
Rmerge	0.081
Total number of observations	44825 *
Number of reflections	13063
Completeness [%]	85.4	57.3 *
Redundancy	3.43

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	50-60 (mg/ml)
2	1	2	Tris-HCl	50 (mM)
3	1	2	2-propanol	12 (%)