1VF7
Crystal structure of the membrane fusion protein, MexA of the multidrug transporter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2003-07-03 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 130.030, 180.350, 214.230 |
| Unit cell angles | 90.00, 106.99, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.400 |
| R-factor | 0.259 |
| Rwork | 0.258 |
| R-free | 0.28220 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.823 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.062 | 0.879 |
| Number of reflections | 365573 | |
| <I/σ(I)> | 26.6 | 1.9 |
| Completeness [%] | 99.2 | |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | HEPES, Tris, glycerol, MPD, PEG 1000, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
