1VF1
cGSTA1-1 in complex with glutathione
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 123 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-03-26 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 55.159, 85.609, 114.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.360 - 1.770 |
| Rwork | 0.192 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.360 | 1.830 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.058 | 0.401 |
| Number of reflections | 27088 | |
| <I/σ(I)> | 8.9 | 2.03 |
| Completeness [%] | 99.0 | 99.3 |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | sodium cacodylate, magnesium acetate, PEG8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
