1VES
Structure of New Antigen Receptor variable domain from sharks
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-10 |
| Detector | MAR scanner 180 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 65.283, 92.046, 98.224 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.120 - 2.180 |
| R-factor | 0.17912 |
| Rwork | 0.176 |
| R-free | 0.24721 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 12Y-1 VNAR PDB ENTRY 1VER |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.484 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.120 | 2.240 |
| High resolution limit [Å] | 2.180 | 2.180 |
| Rmerge | 0.054 | |
| Number of reflections | 14981 | |
| <I/σ(I)> | 32.5 | 4 |
| Completeness [%] | 100.0 | 99.4 |
| Redundancy | 6.6 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 0.1M Sodium citrate, 20% v/v iso-Propanol, 20% PEG4000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
