1VER
Structure of New Antigen Receptor variable domain from sharks
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-10 |
| Detector | MAR scanner 180 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 97.259, 97.259, 65.228 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.820 |
| R-factor | 0.16972 |
| Rwork | 0.166 |
| R-free | 0.25391 |
| Structure solution method | MIR |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.552 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.010 | 2.900 |
| High resolution limit [Å] | 2.820 | 2.820 |
| Rmerge | 0.045 | |
| Number of reflections | 3975 | |
| <I/σ(I)> | 18.1 | 1.8 |
| Completeness [%] | 99.0 | 94.3 |
| Redundancy | 15.1 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1M BIS-TRIS PROPANE, 45% PPG P400, pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
