1VEM

Crystal Structure Analysis of Bacillus Cereus Beta-Amylase at the optimum pH (6.5)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	MACSCIENCE
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	2002-09-02
Detector	SIEMENS HI-STAR
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	57.576, 92.752, 65.787
Unit cell angles	90.00, 102.36, 90.00

Refinement procedure

Resolution	14.930 - 1.850
R-factor	0.176
Rwork	0.176
R-free	0.20500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1b9z
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	SAINT
Data scaling software	SAINT
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.900	1.780
High resolution limit [Å]	1.700	1.720
Number of reflections	63534
Completeness [%]	88.9	44.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	PEG 6000, ammonium sulfate, potassium phosphate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K