1VEC
Crystal structure of the N-terminal domain of rck/p54, a human DEAD-box protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-02-15 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.520, 73.140, 84.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.900 - 2.010 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qde |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.900 | 2.080 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.073 | 0.263 |
| Number of reflections | 52054 | |
| <I/σ(I)> | 7.4 | 2.1 |
| Completeness [%] | 99.3 | 98.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 298 | PEG3350, di-sodium tartrate, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
