1VEA

Crystal Structure of HutP, an RNA binding antitermination protein

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	100
Detector technology	CCD
Collection date	2002-10-03
Detector	ADSC QUANTUM 4
Wavelength(s)	0.978, 1.007, 0.995, 0.950
Spacegroup name	P 21 3
Unit cell lengths	95.410, 95.410, 95.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.480 - 2.800
R-factor	0.23
Rwork	0.230
R-free	0.28100
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.803	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.054	0.258
Number of reflections	7375
<I/σ(I)>	7.9	3.2
Completeness [%]	100.0	100
Redundancy	7.1	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	293	PEG2000MME, Pottasium bromide, Na Cacodylate, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K