1VE5

Crystal Structure of T.th. HB8 Threonine deaminase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	293
Detector technology	CCD
Collection date	2002-01-31
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	53.590, 158.190, 80.300
Unit cell angles	90.00, 108.52, 90.00

Refinement procedure

Resolution	10.000 - 2.150
Rwork	0.202
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.400
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.270
High resolution limit [Å]	2.150	2.150
Number of reflections	65660
Completeness [%]	95.7	95.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	PEG4000, MPD, Ca(Ace)2, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K