1VE3
Crystal structure of PH0226 protein from Pyrococcus horikoshii OT3
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-02-28 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 0.97882, 0.97945, 1.0 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 58.167, 58.167, 252.463 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.330 - 2.100 |
| R-factor | 0.236 |
| Rwork | 0.236 |
| R-free | 0.27000 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.049 | |
| Number of reflections | 29753 | |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 5.2 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 4.6 | 295 | PEG 4K, Na acetate, pH 4.6, microbatch, temperature 295.0K |
