1VCX
Neutron Crystal Structure of the Wild Type Rubredoxin from Pyrococcus Furiosus at 1.5A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | NUCLEAR REACTOR |
| Source details | JRR-3M BEAMLINE 1G-A |
| Synchrotron site | JRR-3M |
| Beamline | 1G-A |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-08-24 |
| Detector | MACSCIENCE DIP100 |
| Wavelength(s) | 2.33 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.320, 35.310, 44.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.600 - 1.500 |
| Rwork | 0.186 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1brf |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.240 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 7417 | |
| <I/σ(I)> | 8.5 | 3.1 |
| Completeness [%] | 81.9 | 53.1 |
| Redundancy | 3.3 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Macroseeding | 8 | 298 | sodium dihydrogen phosphate, dipotassium hydrogen phosphate, pH 8.0, Macroseeding, temperature 298K |
