1VCX
Neutron Crystal Structure of the Wild Type Rubredoxin from Pyrococcus Furiosus at 1.5A Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | NUCLEAR REACTOR |
Source details | JRR-3M BEAMLINE 1G-A |
Synchrotron site | JRR-3M |
Beamline | 1G-A |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 1999-08-24 |
Detector | MACSCIENCE DIP100 |
Wavelength(s) | 2.33 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 34.320, 35.310, 44.230 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.600 - 1.500 |
Rwork | 0.186 |
R-free | 0.21700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1brf |
RMSD bond length | 0.010 |
RMSD bond angle | 1.240 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 7417 | |
<I/σ(I)> | 8.5 | 3.1 |
Completeness [%] | 81.9 | 53.1 |
Redundancy | 3.3 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Macroseeding | 8 | 298 | sodium dihydrogen phosphate, dipotassium hydrogen phosphate, pH 8.0, Macroseeding, temperature 298K |