1VCQ

SEMLIKI FOREST VIRUS CAPSID PROTEIN (CRYSTAL FORM II)

Experimental procedure

Detector technology	AREA DETECTOR
Detector	SIEMENS
Spacegroup name	P 1 21 1
Unit cell lengths	53.717, 48.572, 70.372
Unit cell angles	90.00, 105.77, 90.00

Refinement procedure

Resolution	6.500 - 3.300 *
R-factor	0.181 *
Rwork	0.157
RMSD bond length	0.013
RMSD bond angle	1.700
Data reduction software	XDS
Data scaling software	XDS
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	3.300 *	3.300 *
Rmerge	0.093	0.093 *
Number of reflections	5222	1286 *
Completeness [%]	80.3	80.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.3 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop		5 (mM)
3	1	drop	PEG8000	3-5 (%(w/v))
4	1	drop	Tris-HCl	100-150 (%(w/v))
5	1	reservoir	PEG8000	6-10 (%(w/v))
6	1	reservoir	Tris-HCl	200-300 (mM)