1VCE
Crystal structure of project ID PH0725 from Pyrococcus horikoshii OT3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-17 |
| Detector | RIGAKU JUPITER |
| Wavelength(s) | 0.97938 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 104.721, 104.721, 138.672 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.210 - 2.100 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.25300 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.061 | 0.540 |
| Number of reflections | 45662 | |
| <I/σ(I)> | 13.3 | 3.85 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.6 | 14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microbach | 5.5 | 291 | 3.85M Na Formate, 0.1M Acetate, pH 5.5, microbach, temperature 291K |
