1VCA

CRYSTAL STRUCTURE OF AN INTEGRIN-BINDING FRAGMENT OF VASCULAR CELL ADHESION MOLECULE-1 AT 1.8 ANGSTROMS RESOLUTION

Experimental procedure

Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Detector technology	IMAGE PLATE
Collection date	1994
Detector	FUJI
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	52.700, 66.500, 113.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 1.800
R-factor	0.202
Rwork	0.202
RMSD bond length	0.015
RMSD bond angle	1.990
Data reduction software	DENZO
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
High resolution limit [Å]	1.800 *
Rmerge	0.087 *
Number of reflections	32077
Completeness [%]	85.0
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *			Bottomley, M.J., (1994) J.Mol.Biol., 244, 464. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	13 (mg/ml)
2	1	reservoir	lithium sulphate monohydrate	0.14 (M)
3	1	reservoir	Tris-HCl	0.1 (M)
4	1	reservoir	PEG4000	20-22 (%(w/v))