1VBR
Crystal structure of complex xylanase 10B from Thermotoga maritima with xylobiose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-04 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 58.460, 58.430, 61.290 |
| Unit cell angles | 70.43, 84.33, 68.77 |
Refinement procedure
| Resolution | 29.580 - 1.800 |
| R-factor | 0.194 |
| Rwork | 0.181 |
| R-free | 0.21500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 64332 | |
| Completeness [%] | 100.0 | 96.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.3 | 293 | PEG 8000, sodium acetate, lithium sulfate, pH 4.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
