1VBL
Structure of the thermostable pectate lyase PL 47
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Detector technology | CCD |
Collection date | 2001-10-19 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 58.610, 58.610, 229.420 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.910 |
R-factor | 0.182 |
Rwork | 0.182 |
R-free | 0.20900 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 1.910 |
Number of reflections | 32506 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.7 | PEG 4000, 2-propanol, pH 7.7, VAPOR DIFFUSION, HANGING DROP |