1VAU
Xenon derivative of hen egg-white lysozyme
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-03-29 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.5400, 0.3580, 0.3579 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.330, 78.330, 36.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.500 |
| Rwork | 0.187 |
| R-free | 0.20300 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 18835 | |
| <I/σ(I)> | 38.9 | 4.8 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 11.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 50mg/ml HEWL, 1.8M NaCl, 100mM sodium citrate (pH 4.5), 30%(v/v) glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
