1VAS
ATOMIC MODEL OF A PYRIMIDINE DIMER SPECIFIC EXCISION REPAIR ENZYME COMPLEXED WITH A DNA SUBSTRATE: STRUCTURAL BASIS FOR DAMAGED DNA RECOGNITION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-06-03 |
| Detector | MACSCIENCE DIP100 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 65 |
| Unit cell lengths | 118.820, 118.820, 36.280 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.750 |
| R-factor | 0.152 |
| Rwork | 0.152 |
| R-free | 0.24000 |
| RMSD bond length | 0.015 * |
| RMSD bond angle | 1.980 * |
| Data reduction software | ELMS |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.750 |
| Rmerge | 0.062 |
| Total number of observations | 24907 * |
| Number of reflections | 5662 |
| Completeness [%] | 72.0 |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 4 * | pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 277.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | BIS-TRIS-PROPANE_HCL | ||
| 3 | 1 | 1 | NH4 SULFATE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.38 (mM) | |
| 2 | 1 | drop | DNA | 0.53 (mM) | duplex |
| 3 | 1 | drop | Tris-HCl | 150 (mM) | |
| 4 | 1 | drop | ammonium sulfate | 33 (%sat) | |
| 5 | 1 | reservoir | ammonium sulfate | 54 (%sat) |
