1VAP

THE MONOMERIC ASP49 SECRETORY PHOSPHOLIPASE A2 FROM THE VENOM OF AGKISTRIDON PISCIVORUS PISCIVORUS

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1990-12-04
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 1
Unit cell lengths	34.710, 39.890, 42.850
Unit cell angles	94.17, 79.10, 106.27

Refinement procedure

Resolution	10.000 - 1.600
R-factor	0.197 *
Rwork	0.197
RMSD bond length	0.010
Data reduction software	HAMLIN
Phasing software	X-PLOR
Refinement software	PROFFT

Data quality characteristics

	Overall
Low resolution limit [Å]	99.000 *
High resolution limit [Å]	1.500 *
Rmerge	0.042
Number of reflections	28084
Completeness [%]	82.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	App-D49	0.03 (mg/ml)
2	1	drop	ethanol	20 (%(v/v))
3	1	drop	Tris-HCl	0.1 (M)
4	1	reservoir	ethanol	40 (%(v/v))
5	1	reservoir	Tris-HCl	0.2 (M)