1VAO
STRUCTURE OF THE OCTAMERIC FLAVOENZYME VANILLYL-ALCOHOL OXIDASE
Experimental procedure
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE AREA DETECTOR |
Collection date | 1996-10 |
Detector | MARRESEARCH |
Spacegroup name | I 4 |
Unit cell lengths | 130.240, 130.240, 133.510 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.500 |
R-factor | 0.221 |
Rwork | 0.220 |
R-free | 0.29700 * |
Structure solution method | MIR+MR+DENSITY AVERAGING |
Starting model (for MR) | P-CRESOL METHYLHYDROXYLASE |
RMSD bond length | 0.016 |
RMSD bond angle | 20.890 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CCP4 |
Refinement software | TNT (5E) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.088 * | 0.210 * |
Total number of observations | 330030 * | |
Number of reflections | 38274 | |
<I/σ(I)> | 9 | 3 |
Completeness [%] | 98.9 | 98.3 |
Redundancy | 10 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4.6 | FROM 6% PEG4000, 100 MM ACETATE BUFFER PH 4.6 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG4000 | 6 (%(w/v)) | |
2 | 1 | reservoir | sodium acetate/HCl | 0.1 (M) |