1VAM

CONCANAVALIN A COMPLEX WITH 4'-NITROPHENYL-ALPHA-D-MANNOPYRANOSIDE

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1994-04-15
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	135.190, 155.380, 71.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.750
R-factor	0.185
Rwork	0.185
R-free	0.26000
RMSD bond length	0.009
RMSD bond angle	26.600 *
Data reduction software	XDS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.000
High resolution limit [Å]	2.750	2.750 *
Rmerge	0.125	0.584 *
Total number of observations	181088 *
Number of reflections	38810
Completeness [%]	97.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris	10 (mM)
2	1	reservoir	ammonium sulfate	1 (M)
3	1	drop	ConA	70 (mg/ml)
4	1	drop	saccharide		10 * molar excess