1VA7
Yeast Myo3 SH3 domain, triclinic crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8115 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.800, 48.750, 48.840 |
| Unit cell angles | 60.70, 70.74, 70.60 |
Refinement procedure
| Resolution | 20.000 - 2.900 |
| R-factor | 0.23831 |
| Rwork | 0.236 |
| R-free | 0.28017 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ruw |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.294 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 6221 | |
| <I/σ(I)> | 7.1 | 2.5 |
| Completeness [%] | 96.7 | 96.8 |
| Redundancy | 2.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | sodium citrate, glycerol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
