1V9U

Human Rhinovirus 2 bound to a fragment of its cellular receptor protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2002-07-09
Detector	ADSC QUANTUM 4
Wavelength(s)	0.939
Spacegroup name	P 21 2 21
Unit cell lengths	313.100, 348.790, 380.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 3.600
Rwork	0.285
R-free	0.29500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fpn
RMSD bond length	0.013
RMSD bond angle	1.630
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	3.500
Rmerge	0.128
Number of reflections	362883
<I/σ(I)>	4
Completeness [%]	71.9
Redundancy	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	Ammonim sulfate, sodium/potassium phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K