1V9D

Crystal structure of the core FH2 domain of mouse mDia1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-04-06
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9340
Spacegroup name	P 21 21 21
Unit cell lengths	82.430, 124.521, 229.404
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.120 - 2.600
R-factor	0.239
Rwork	0.236
R-free	0.26680 *
Structure solution method	SAD
RMSD bond length	0.006 *
RMSD bond angle	1.120 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.099 *	0.495 *
Total number of observations	406569 *
Number of reflections	73264
Completeness [%]	99.6	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.3	20 *	PEG3350, sodium sulfate, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	PEG3350	20 (%(v/v))
3	1	reservoir		200 (mM)