1V8X
Crystal Structure of the Dioxygen-bound Heme Oxygenase from Corynebacterium diphtheriae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-10-25 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.003, 62.969, 107.490 |
| Unit cell angles | 90.00, 100.96, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.850 |
| R-factor | 0.15705 |
| Rwork | 0.153 |
| R-free | 0.19252 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.781 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 60627 | |
| <I/σ(I)> | 22 | 4.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.1 | 303 | MES, Ammonium sulfate, pH 6.1, VAPOR DIFFUSION, temperature 303K |
