1V8T

Crystal Structure analysis of the ADP-ribose pyrophosphatase complexed with ribose-5'-phosphate and Zn

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS
Wavelength(s)	1.54
Spacegroup name	P 32 2 1
Unit cell lengths	49.930, 49.930, 117.950
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.320 - 2.600
R-factor	0.22
Rwork	0.220
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.700
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.690
High resolution limit [Å]	2.600	2.600
Number of reflections	5224
Completeness [%]	93.4	85.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	VAPOR DIFFUSION, HANGING DROP, temperature 293K