1V8D
Crystal structure of the conserved hypothetical protein TT1679 from Thermus thermophilus
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44B2 |
Synchrotron site | SPring-8 |
Beamline | BL44B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-03-08 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9796, 0.9799, 0.9819, 0.9744 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 102.799, 115.224, 75.270 |
Unit cell angles | 90.00, 129.76, 90.00 |
Refinement procedure
Resolution | 34.550 - 2.160 |
R-factor | 0.189 |
Rwork | 0.189 |
R-free | 0.24900 |
Structure solution method | MAD |
RMSD bond length | 0.028 |
RMSD bond angle | 2.400 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.230 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.044 | 0.095 |
Number of reflections | 32472 | |
<I/σ(I)> | 17.3 | |
Completeness [%] | 93.1 | 73 |
Redundancy | 2.4 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | PEG3350, MPD, Sodium Thiocyanate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | PEG3350, MPD, Sodium Thiocyanate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |