1V7O

Alanyl-tRNA synthetase editing domain homologue protein from Pyrococcus horikoshii

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-10-18
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9797, 0.9800, 0.9000
Spacegroup name	P 21 21 21
Unit cell lengths	34.191, 88.741, 110.122
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.620
Rwork	0.235
R-free	0.27300
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.400 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.710
High resolution limit [Å]	2.620	2.620
Rmerge	0.081	0.394
Number of reflections	10576
<I/σ(I)>	9.2
Completeness [%]	99.7	99.6
Redundancy	6.3	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.6 *	20 *	PEG 4000, Tris, MPD, pH 8.8, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris-HCl	0.1 (M)	pH8.6
2	1	reservoir	PEG4000	24 (%(w/v))
3	1	reservoir	MPD	3 (%(v/v))