1V6S
Crystal Structure of Phosphoglycerate Kinase from Thermus thermophilus HB8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-10-18 |
Detector | RIGAKU RAXIS V |
Wavelength(s) | 1.00 |
Spacegroup name | P 65 |
Unit cell lengths | 143.175, 143.175, 75.641 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 1.500 |
R-factor | 0.202 |
Rwork | 0.201 |
R-free | 0.21500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vpe |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.051 | 0.192 |
Number of reflections | 140815 | |
<I/σ(I)> | 21.4 | 7.89 |
Completeness [%] | 99.9 | 99.3 |
Redundancy | 7.46 | 5.38 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 5.3 | 291 | sodium formate, acetate, pH 5.3, microbatch, temperature 291K |