1V6L
Peanut lectin-lactose complex in the presence of 9mer peptide (PVIWSSATG)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 128.278, 124.636, 75.752 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.173 |
| Rwork | 0.173 |
| R-free | 0.22400 * |
| Structure solution method | isomorphous replacement |
| RMSD bond length | 0.014 |
| RMSD bond angle | 26.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.520 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.127 | 0.461 |
| Total number of observations | 120907 * | |
| Number of reflections | 41998 | 2143 * |
| Completeness [%] | 96.5 * | 98.5 * |
| Redundancy | 2.9 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.6 | 293 | PEG 8000, 0.2M calcium acetate, 0.1M cacodylate pH 6.5, pH 4.6, Hanging drop method, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4.2 (mg/ml) | |
| 2 | 1 | drop | sodium acetate | 0.05 (M) | |
| 3 | 1 | drop | 0.2 (M) | ||
| 4 | 1 | drop | sodium azide | 0.05 (%) | |
| 5 | 1 | drop | lactose | 2.1 (mM) | |
| 6 | 1 | reservoir | PEG8000 | 12 (%) |
