1V6D
The crystal structure of the trypsin complex with synthetic heterochiral peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-08-09 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.930, 53.719, 77.473 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.280 - 1.900 |
| R-factor | 0.15453 |
| Rwork | 0.153 |
| R-free | 0.17849 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qqu |
| RMSD bond length | 0.024 |
| RMSD bond angle | 3.554 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.280 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.069 | |
| Number of reflections | 15129 | |
| Completeness [%] | 94.2 | 94.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 293 | 2M ammonium sulphate, pH 6.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
