1V6C
Crystal Structure of Psychrophilic Subtilisin-like Protease Apa1 from Antarctic Psychrotroph Pseudoalteromonas sp. AS-11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL40B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL40B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-04 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 122.942, 138.476, 64.772 |
| Unit cell angles | 90.00, 97.48, 90.00 |
Refinement procedure
| Resolution | 36.620 - 1.800 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.21500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bh6 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.750 | 1.840 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.077 | 0.317 |
| Number of reflections | 101031 | |
| Completeness [%] | 98.2 | 94.1 |
| Redundancy | 7.5 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 30% PEG5000MME, 0.1M sodium sulfate, 0.1M Tris, 1mM calcium chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
