1V5C
The crystal structure of the inactive form chitosanase from Bacillus sp. K17 at pH3.7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-10 |
| Detector | OXFORD PX210 |
| Wavelength(s) | 0.90 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 85.063, 91.145, 131.524 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.510 - 2.000 |
| Rwork | 0.184 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.510 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.072 | 0.275 |
| Number of reflections | 34863 | |
| <I/σ(I)> | 9.4 | 3 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 7.19 | 7.39 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.7 | 277 | PEG 4000, ammonium sulfate, sodium citrate, pH 3.7, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
