1V4X
Crystal structure of bluefin tuna hemoglobin deoxy form at pH5.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL40B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL40B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.979, 60.537, 80.640 |
| Unit cell angles | 90.00, 95.12, 90.00 |
Refinement procedure
| Resolution | 19.730 - 1.600 |
| R-factor | 0.20587 |
| Rwork | 0.204 |
| R-free | 0.25030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.748 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.22) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.073 | 0.331 |
| Number of reflections | 63648 | |
| <I/σ(I)> | 9.8 | |
| Completeness [%] | 88.4 | 72.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH METHOD | 5 | 293 | PEG 2000, sodium phosphate, magnesium chloride, pH 5.0, BATCH METHOD, temperature 293K |
