1V4P
Crystal structure of Alanyl-tRNA Synthetase from Pyrococcus horikoshii OT3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-10-03 |
Detector | RIGAKU RAXIS V |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.802, 97.861, 60.872 |
Unit cell angles | 90.00, 96.51, 90.00 |
Refinement procedure
Resolution | 38.040 - 1.450 |
R-factor | 0.207 |
Rwork | 0.206 |
R-free | 0.22700 |
Structure solution method | MAD |
RMSD bond length | 0.004 |
RMSD bond angle | 1.297 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.500 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.051 | 0.453 |
Number of reflections | 88112 | |
<I/σ(I)> | 2.3 | |
Completeness [%] | 99.9 | 99.5 |
Redundancy | 3.7 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 6.3 | 291 | PEG 4000, pH 6.3, Microbatch, temperature 291K |
1 | MICROBATCH | 6.3 | 291 | PEG 4000, pH 6.3, Microbatch, temperature 291K |