1V4J
Crystal Structure of Octaprenyl Pyrophosphate Synthase from Hyperthermophilic Thermotoga maritima V73Y mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.54 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 150.812, 150.812, 68.864 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.850 |
Rwork | 0.237 |
R-free | 0.27420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1v4e |
RMSD bond length | 0.004 |
RMSD bond angle | 0.997 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.950 |
High resolution limit [Å] | 2.850 | 2.850 |
Rmerge | 0.070 | 0.598 |
Total number of observations | 129154 * | |
Number of reflections | 18359 | |
<I/σ(I)> | 16.96 | 3.57 |
Completeness [%] | 99.9 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | Na+Hepes, lithium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | Triton X-100 | 0.1 (%) | |
3 | 1 | reservoir | sodium HEPES | 0.1 (M) | pH7.5 |
4 | 1 | reservoir | 1.5 (M) |