1V3T
Crystal structure of leukotriene B4 12-hydroxydehydrogenase/15-oxo-prostaglandin 13-reductase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44B2 |
Synchrotron site | SPring-8 |
Beamline | BL44B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-05-27 |
Detector | MARRESEARCH |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.748, 77.071, 79.601 |
Unit cell angles | 90.00, 102.73, 90.00 |
Refinement procedure
Resolution | 24.500 - 2.300 |
Rwork | 0.194 |
R-free | 0.25520 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.006 |
RMSD bond angle | 1.282 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.500 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.048 | 0.123 |
Number of reflections | 29190 | |
<I/σ(I)> | 32.2 | 14.9 |
Completeness [%] | 94.9 | 99.3 |
Redundancy | 8.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | batch | 6.5 | 291 | PEG 4000, MES, magnesium chloride, pH 6.5, batch, temperature 291K |